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My current research activities center around the role of membrane proteins cellular growth and tissue differentiation. The milk fat globule membrane is used as a model for these studies. Cell and tissue culture, cell separation, immunocytochemistry, monoclonal antibody production, radioisotope spectrometry, thin layer and column chromatography of steroids and proteins, autoradiography of steroids, differential and sucrose density gradient centrifugation, radioimmunoassay, polyclonal antibody preparation and purification, acrylamide electrophoresis of proteins, extraction and purification of DNA from cells, digestion with restriction enzymes, ligating of vectors and foreign DNA, transformation of procaryotic and eucaryotic cells, analysis of recombinant clones, PCR, DNA sequencing, production of genomic DNA libraries and expression of inserted genes in eucaryotic cells, and general analytical instrumentation involved in immunology, microbiology, physiology and biochemistry and competitive hormone binding assays, light and electron microscopy.
Surgical procedures are utilized such as blood vessel cannulation, hysterectomy, ovariectomy and liver and mammary gland biopsy.
Electromagnetic, ultrasound and laser-doppler methods have been used to measure blood flow in dairy cattle in response to various physiological, pathophysiological and pharmaco-logical stimuli. Computerized data acquisition systems are employed with these systems.
- mammary gland
- tissue engineering
submitted impact statement
selected publications listing
Gorewit, R.C., and H.A. Tucker. Corticoid binding in mammary tissue slices from lactating cows. J. Dairy Science 59:232-240, 1976.
Gorewit, R.C., and H.A. Tucker. Comparison of binding proteins of glucocorticoids in mammary tissue and in blood sera from lactating cows. J. Dairy Sci. 59:1247-1253, 1976.
Gorewit, R.C., and H.A. Tucker. Glucocorticoid binding in mammary tissue slices of cattle in various reproductive states. J. Dairy Science 59:1890-1896, 1976.
Gorewit, R.C., and H.A. Tucker. Lactational events related to glucocorticoid binding in bovine mammary tissue. J. Dairy Science 60:889-895, 1977.
Stellflug, J.N., T.M. Louis, R.C. Gorewit, W.D. Oxender and H.D. Hafs. Luteolysis induced by prostaglandin F2alpha before and after hysterectomy in heifers. Biol. Reprod. 17:535-540, 1977.
Gorewit, R.C. Method for determining oxytocin concentrations in unextracted sera, characterization in lactating cattle. Proc. Soc. Exp. Biol. and Med. 160:80-87, 1979.
Kleinman, L.I., R.O. Banks and R.C. Gorewit. Role of oxytocin in naturesis of the rat. J. of Pediatrics 96:159, 1980.
Sagi, R., R.C. Gorewit, W.G. Merrill and D.B. Wilson. Premilking stimulation effects on milking performance and oxytocin and prolactin release in cows. J. Dairy Science 63:800-806, 1980.
Sagi, R., R.C. Gorewit, and D.B. Wilson. Role of exogenous oxytocin in eliciting milk ejection in dairy cows. J. Dairy Sci. 63:2006- 2011, 1980.
Sagi, R., R.C. Gorewit and S.A. Zinn. Milk ejection in cows mechanically stimulated during late lactation. J. Dairy Sci. 63:1957-1060, 1980.
Gorewit, R.C. Effects of clonidine on glucose production and insulin secretion in cattle. J. Vet. Res. 41:1769-1772, 1980.
Wilson, D.B., and R.C. Gorewit. Specific thyroxine receptors in mammary cytosol from lactating cattle. Biochem. Biophys. Res. Comm. 95:807-815, 1980.
Gorewit, R.C. Influence of somatostatin on serum prolactin concentrations of cows during rest and milking. Experientia 36:359-361, 1980.
Gorewit, R.C. Pituitary thyroid and adrenal response to clonidine in dairy cattle. J. Endocrinol. Invest. 4:135-139, 1981.
Peel, C.J., D.E. Bauman, R.C. Gorewit and C.J. Sniffen. Effect of exogenous growth hormone on lactational performance in high yielding dairy cows. J. Nutrition 3:1662-1671, 1981.
Gorewit, R.C. Effect of apomorphine hydrochloride administration on serum concentrations of prolactin and growth hormone in cattle. Experientia 37:204-205, 1981.
Burden, H.W., R.C. Gorewit, T.M. Louis, P.D. Muse and I.E. Lawrence. Plasma oxytocin and estradiol in pelvic neurectomized rats with blocked parturition. Endokrinologie Band 79, Heft 3.S 379-384, 1982.
Blackwell, J., P. McKercher, F. Kosikowski, L. Carmichael and R.C. Gorewit. Concentration of foot and mouth disease virus in milk of cows infected under simulated field conditions. J. Dairy Science 65:1624-1631 1982.
Zinn, S.A., R.C. Gorewit and R. Sagi. Milking responses of cows given premilking stimulation at four lactational stages. J. Dairy Sci. 65:668-671, 1982.
Peel, C.J., T.J. Fronk, D.E. Bauman, and R.C. Gorewit. Lactational response to exogenous growth hormone and abomasal infusion of a glucose-sodium caseinate mixture in high yield dairy cows. J. Nutri. 112:1170-1178, 1982.
Gorewit, R.C., R. Sagi, E.A. Wachs and W.G. Merrill. Current concepts on the role of oxytocin in milk ejection. Symposium. J. Dairy Sci. 66:2236-2250, 1983.
Fronk, T.J., C.J. Peel, D.E. Bauman and R.C. Gorewit. Comparison of different patterns of exogenous growth hormone administration on milk production in holstein cows. J. Anim. Sci. 57:699-705, 1983.
Gorewit, R.C. Pituitary and thyroid responses of heifers after ferulic acid administration. J. Dairy Sci. 66:624-629 1983.
Blackwell, J., P. McKercher, F. Kosikowski, L. Carmichael and R.C. Gorewit. Physiochemical transformation of milk components and release of foot and mouth disease virus. J. Dairy Research. 50:17-25, 1983.
Thompson, P.D., R.E. Pearson and R.C. Gorewit. Milking cows with positive pressure stimulation in late lactation. J. Dairy Sci. 66:1167-1173, 1983.
Peel, C.J., T.J. Fronk, D.E. Bauman and R.C. Gorewit. Growth hormone administration and abomasal infusion of casein and glucose on lactational performance of dairy cows. J. Nutrition 112:1770-1778, 1983.
Peel, C.J., T.J. Fronk, D.E. Bauman and R.C. Gorewit. Effect of exogenous growth hormone in early and late lactation on lactational performance in dairy cows. J. Dairy Sci. 66:776-782, 1983.
Blackwell, J., P. McKercher, F. Kosikowski, L. Carmichael and R.C. Gorewit. Histological and histochemical characterization of mammary gland tissue of cows infected with foot-and-mouth disease by contact exposure. Res. in Vet. Sci. 35:106-113, 1983.
Wachs, E.A., R.C. Gorewit and W.B. Currie. Half-life, clearance, and production rate for oxytocin in cattle during lactation and mammary involution. I. Domestic Animal Endocrinology 1:121-140, 1984.
Wachs, E.A., R.C. Gorewit and W.B. Currie. Oxytocin concentrations of cattle in response to milking stimuli through lactation and involution. II. Domestic Animal Endocrinology 1:141-154, 1984.
Gorewit, R.C. Physiological and pharmacological studies of oxytocin in virgin cattle. III. Domestic Animal Endocrinology 1:155-165, 1984.
Gorewit, R.C. and R. Sagi. Effects of exogenous oxytocin on production and milking variables of cows. J. Dairy Science 67:2050-2054, 1984.
Gorewit, R.C., N.R. Scott and C.S. Czarniecki. Responses of dairy cows to alternating electrical current administered semi-randomly in a non-avoidance environment. J. Dairy Sci. 68:718-725, 1985.
Gorewit, R. C. and K.B. Gassman. Effects of duration of udder stimulation on milking dynamics and oxytocin release. J. Dairy Sci. 68:1813-1818, 1985.
Drenkard, D.V., R.C. Gorewit, R. Sagi and N.R. Scott. Milk production, health and endocrine responses of cows exposed to electrical current during milking. J. Dairy Sci. 68:2694-2702, 1985.
Gorewit, R.C. and M.C. Aromando. Mechanisms involved in the adrenalin-induced blockade of milk ejection in dairy cattle. Proc. Soc. Exp. Biol. Med. 180:340-347, 1985.
Gorewit, R.C. and N.R. Scott. Cardiovascular responses of cows given electrical current during milking. J. Dairy Sci. 69:1122-1127, 1986.
Zhao, Xin and R.C. Gorewit. Oxytocin receptors in bovine mammary tissue. J. Recept. Res. 7:729-741, 1987.
Zhao, Xin and R.C. Gorewit. Inositol-phosphate response to oxytocin stimulation in dispersed bovine mammary cells. Neuropeptides 10:227-233, 1987.
Gorewit, R.C., H.Y. Chen and J.J. Kopchick. Growth hormone gene expression in myoepithelial cells directed by various eucaryotic transcriptional regulatory sequences. FEBS Let. 225:238-242, 1987.
Currie, W.B., R.C. Gorewit and F.J. Michel. Endocrine changes, with special emphasis on oestradiol-17B, prolactin and oxytocin, before and during labour and delivery in goats. J. Reprod. Fert. 82:299-308, 1988.
Gorewit, R.C., M.C. Aromando and D.G. Bristol. Measuring mammary gland blood flow using a transit-time ultrasonic flow probe. J. Dairy Sci. 72:1918-1928, 1989.
Gorewit, R.C., D.J. Aneshansley, D.C. Ludington, R.A. Pellerin and Xin Zhao. AC voltages on water bowls: effects on lactating Holsteins. J. Dairy Sci. 72:2184-2192, 1989.
Zhao, Xin, R.C. Gorewit and W.B. Currie. Effects of N-acetylimadizole on oxytocin binding in mammary tissue. J. Recep. Res, 10:287-298, 1990.
Zhao, Xin and R.C. Gorewit. Characterization of vasopressin binding sites in bovine mammary tissue. Neuropeptides (Life Science Advances): 10: 1-8, 1991.
Gorewit, R.C., K. Svennersten, W.R. Butler, and K. Uvnas-Moberg. Endocrine responses in cows milked by hand and machine. J. Dairy Sci. 75:443-448, 1992.
Aneshansley, D.J., R.C. Gorewit and L.R. Price. Cow Sensitivity to Electricity during Milking. J. Dairy Sci. 75:2733-2741,1992.
R. C. Gorewit, D.J. Aneshansley, and L. R. Price. Effects of Voltages on Cows over a complete lactation. 1. Milk Yield and Composition. J. Dairy Sci. 75:2719-2725, 1992.
R. C. Gorewit, D. J. Aneshansley and L. R. Price. Effects of voltages on cows over a complete lactation. 2. Health and Reproduction. J. Dairy Sci. 75:2726-2732, 1992.
Politis I., R.C.Gorewit, T. Muller and R. Grosse. Mammary-derived growth inhibitor protein and messenger ribonucleic acid concentrations in different physiological states of the gland. J. Dairy Sci. 75:1423-1429, 1992.
Politis, I., R.C. Gorewit, T. Muller and R. Grosse. Comparison of mammary-derived growth inhibitor production in mammary tissue of lactating pregnant versus nonlactating-pregnant holstein cows. Can. J. Anim. Sci.72:417-420, 1992.
Politis, I., R. C. Gorewit, T. Muller and R. Grosse. Mammary-derived growth inhibitor in lactation and involution. Domestic Animal Endocrinology 9:89-94, 1992.
Politis, I., D. M. Barbano and R. C. Gorewit. Distribution of plasminogen and plasmin in fractions of bovine milk. J. Dairy Sci. 75:1402-1410, 1992.
T. Zachos, I. Politis, R. C. Gorewit and D. Barbano. Effect of mastitis on plasminogen activator activity of milk somatic cells. J. Dairy Research 59:461-467, 1992.
Politis, I., and R. C. Gorewit. Comparison of functional properties of mammary macrophages isolated from mastitic and healthy quarters. Animal Science Review 9:86-94, 1992.
Zavizion, B., I. Politis and R. C. Gorewit. Bovine Mammary Myoepithelial cells. 1. Isolation, culture and characterization. J. Dairy Sci. 75:3367-3380, 1992.
Zavizion, B., I. Politis and R. C. Gorewit. Bovine mammary myoepithelial cells. 2. Interactions with epithelial cells in vitro. J. Dairy Sci. 75:3381-3393, 1992.
Gorewit, R. C., J. Jiang and D. J. Aneshansley. Responses of the bovine mammary artery to angiotensins. J. Dairy Sci. 76:1278-1284, 1993.
Gorewit, R. C. Effects of endotoxin induced mastitis on milking release of oxytocin. J. Dairy Sci. 76:722-727, 1993.
Politis I., B. Zavizion, D. M. Barbano. and R. C. Gorewit. Enzymatic assay for the combined determination of plasmin plus plasminogen in milk: revisited. J. Dairy. Sci. 76:1260:1267, 1993.
Gorewit, R. C., K. Ostennson, G. Astrom, and K. Svennersten. Flow and composition of afferent mammary gland lymph. J. Dairy Sci. 76:1539-1543, 1993
Zavizion, B., I. Politis and R. C. Gorewit. Effects of mammary derived growth inhibitor on proliferation of myoepithelial cells in vitro. Can. J. Anim. Sci. 73:449-452. 1993
Zavizion, B., I. Politis, R. Grosse, J. D. Turner, E. Spitzer and R. C. Gorewit. Effects of mammary- derived growth inhibitor (MDGI) on proliferation of MAC-T bovine mammary epithelial cells. J. Dairy Sci. 76:3721. 1993
Zavizion, B., R.C. Gorewit, and I. Politis. Subcloning the MAC-T bovine mammary epithelial cell line: morphology, growth properties and cytogenetic analysis of clonal cells. J. Dairy Sci. 78:515-527. 1995.
Spitsberg, V., Matitashvili, E. and R.C. Gorewit. Association and coexpression of fatty acid binding protein and glycoprotein CD36 in the bovine mammary gland. Eur. J Biochem. 230:872-878. 1995.
Politis, I., M. Hidiroglou, T.R. Barta, J.A. Gilmore, R.C. Gorewit, and H. Scherf. Effects of vitamin E on immune function of dairy cows. Amer. J. Vet Res. 56:179. 1995.
Svennersten, K., R.C. Gorewit, L.O. Sjaunja and K. Uvnas-Moberg. Feeding during milking enhances milking-related oxytocin secretion and milk production in dairy cows whereas food deprivation decreases it. Acta Physiologica, Scandinavica. 153:309-310. 1995.
Sammuelsson, B., Uvnaes-Moberg, K., Gorewit, R.C., Svennersten-Sjaunja, K. Profiles of the hormones somatostatin, gastrin, CCK, prolactin, growth hormone, oxytocin and cortisol. I. In dairy cows that are milked and fed separately or milked and fed simultaneously. Livestock Production Science, 46(1)49-56. 1996.
Chung, Y. and Gorewit, R.C. Cloning and Expression of Bovine BRCA1. Pakistan. Journal of Nutrition 1(1)56-63.2002.
Chung, Y. and Gorewit, R.C. Expression of Bovine Mammary Gland SMAD4 and its Relationship to BRCA1.. Pakistan. Journal of Nutrition, 1(1)53-55. 2002
Gorewit, R.C. Relationship between Oxytocin Concentrations, Mammary Blood Flow, Feed and Water Intake, and Milk Yields of Lactating Cows Through the Estrous Cycle. 2002. Journal of Animal and Veterinary Advances. 2(5) 36-43. 2002
Spitsberg, V., and R.C. Gorewit Isolation, Purification and Characterization of Fatty-Acid-Binding Protein from Milk Fat Globule Membrane: Effect of Bovine Growth Hormone Treatment. Pakistan Journal of Nutrition, 1(1) 53. 1(1) : 43. 2002
Chung, Y. and Gorewit, R.C. 2002 .Effects of Estrogen and Prolactin on Bovine BRCA1 Gene Expression. P. Journal of Nutrition 1(1) : 49. 2002
Gorewit, R.C., and D.J. Aneshansley, Effects of Magnetic Fields on Interleukin 1 Production by Bovine Blood Monocytes. Journal of Animal and Veterinary Advances 3(8) 29-36. 2003.
Rivas, A. L. Tadevosyan , R, Gorewit, R.C. Anderson, K. L., Lyman, R. and González, R. N. Relationships between bovine mammry gland phagocyte subpopulations and somatic cell counts. Canadian Journal of Veterinary Research 70:68-74, 2006
B.S. - University of California at Irvine, 1965-1969 Bachelors of Science - Biological Sciences - Area of concentration: microbial genetics - invertebrate zoology
M.S. - Michigan State University, E. Lansing, Michigan 1970-1971 - Masters of Science - Microbiology and Public Health (immunoparisitology, general microbiology)
Ph.D. - Michigan State University, E. Lansing, Michigan 1972-1975 Doctor. of Philosophy - Major, Physiology; minor, pharmacology and biochemistry
awards and distinctions listing
- NIH Predoctoral Fellow
- Professor of The Year, Cornell University Class of 1979
- Ph.D. Opponent, Swedish Agricultural University, 2000