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Worobo, Randy W.
Cornell Faculty Member
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Positions
- Professor, Food Science & Technology at Geneva
Alternative approaches to enhance the safety and quality of food is the primary objective of my research. Long- term research projects include the chemical and genetic characterization of antimicrobial peptides (bacteriocins) produced by bacteria. These peptides have a broad range of hosts that include pathogenic Gram positive and negative bacteria as well as heat resistant molds and bacteria. It is believed that these peptides are self-defense mechanisms to protect limited food supplies in the environment. The ultimate application of these peptides to foods will enhance the safety and quality through the inhibition of pathogens and spoilage microorganisms that may be present in the food. Short term research includes the investigation of new technologies and combinations with existing food processing technologies to enhance the safety and quality. Presently, ultraviolet light, dimethyl dicarbonate and chlorine dioxide are being investigated for their potential application to various foods at different stages of food production. These non-thermal food processing treatments enhance the quality and are being evaluated for their effectiveness in achieving a safe food product. My primary outreach is focused on the fruit, vegetable and beverage industries in New York State. I provide my expertise in sanitation, processing recommendations for products that have problematic heat resistant molds and bacteria spoilage as well as answering basic food safety questions. I am actively involved in Juice HACCP training for the juice industry in New York State as well as the rest of the US. My outreach program is accomplished through workshops, conferences and direct contact with food processors.
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Affiliations
Extension
has primary specialization area
has secondary specialization area
Research
research overview
- The primary focus of my research is focused on enhancing the safety of fruits and vegetables throughout the produce supply chain. The safety of irrigation water used for produce production, pathogen survival in manure amended soil and effective decontamination methods are current research interest areas. Continuing research projects include the investigation of new technologies and combinations with existing food processing technologies to enhance the safety and quality of fruit and vegetable based foods. Part of this research involves non-thermal processing treatments to enhance the safety of high risk foods that include fruit juices and produce. More basic research projects focus on the chemical and genetic characterization of antimicrobial peptides (bacteriocins) produced by bacteria. These peptides have a broad range of hosts that include pathogenic Gram positive and negative bacteria as well as heat resistant molds and bacteria. It is believed that these peptides are self-defense mechanisms to protect limited food supplies in the environment. The ultimate application of these peptides to foods will enhance the safety and quality through the inhibition of pathogens and spoilage microorganisms that may be present in the food.
research activities
principal investigator on
- CONTROL OF HEPATITIS A VIRUS AND E. COLI O157:H7 IN GREEN ONIONS AND SPINACH awarded by NATIONAL INSTITUTE OF FOOD AND AGRICULTURE-USDA 2008 - 2012
- INACTIVATION OF ENTERIC FOODBORNE VIRUSES IN HIGH RISK FOODS BY NON-THERMAL PROCESSING TECHNOLOGIES awarded by NATIONAL INSTITUTE OF FOOD AND AGRICULTURE-USDA 2011 - 2016
- MINIMIZING MICROBIAL FOOD SAFETY HAZARDS OF FRESH AND FRESH-CUT FRUITS AND VEGETABLES THROUGH A FARM TO TABLE APPROACH awarded by NATIONAL INSTITUTE OF FOOD AND AGRICULTURE-USDA 2008 - 2013
co-principal investigator on
area(s) of concentration/expertise
keywords
- Bacteriocins
- Food Safety
- Fruits and Vegetable Safety
- Juice HACCP
- Produce
- Ultraviolet Processing
submitted impact statement
- Improving the safety of a new infant food product through direct assistance
- Ensuring the safety of apple cider for New York State
- Ensuring the safety and healthfulness of fruit juices
- Enhancing the microbial safety of fruits and vegetables.
- Educating consumers about food safety risks associated with children`s sippy cups
Publications
individual publications
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academic article
- Landscape and Meteorological Factors Affecting Prevalence of Three Foodborne Pathogens in Fruit and Vegetable Farms. . Applied Environmental Microbiology. 79:588-600. 2013
- Shelf-life evaluation of natural antimicrobials for Concord and Niagara grape juices.. Journal of Food Protection. 76:72-78. 2013
- The Effect of Micro- and Nanoscale Topography on the Adhesion of Bacterial Cells to Solid Surfaces.. Applied and Environmental Microbiology. 79:2703-12. 2013
- Decontamination of green onions and baby spinach by vaporized ethyl pyruvate. Journal of Food Protection. 75:1012-1022. 2012
- Efficacy of UV, acidified sodium hypochlorite, and mild heat for decontamination of surface and infiltrated Escherichia coli O157:H7 on green onions and baby spinach. Journal of Food Protection. 75:1198-206. 2012
- Functional assignment of YvgO, a novel set of purified and chemically characterized proteinaceous antifungal variants produced by Bacillus thuringiensis SF361. . Applied Environmental Microbiology. 78:2543-2552. 2012
- Growth Inhibition of Foodborne Pathogens by Oenococcus oeni.. Journal of Food Science. 77. 2012
- Oomycete and bacterial pathogens in New York surface irrigation water: Survey results and ultraviolet treatment.. Phytopathology. 102. 2012
- Patulin Reduction in Apple Juice from Concentrate by UV Radiation and Comparison of Kinetic Degradation Models between Apple Juice and Apple Cider. Journal of Food Protection. 75:717-724. 2012
- Reducing Patulin Contamination in Apple Juice by Using Inactive Yeast. Journal of Food Protection. 74:149-153. 2011
- The 3D solution structure of thurincin H, a bacteriocin with four sulfur to α-carbon crosslinks. . Angew Chem Int Ed Engl.. 50:8718-21. 2011
- Identification and haplotype distribution of Alicyclobacillus spp. from different juices and beverages. International Journal of Food Microbiology. 142:286-291. 2010
- Reduction of patulin in apple cider by ultraviolet light. Journal of Food Protection. 73:69-74. 2010
- Thermoaciduric Clostridium pasteurianum Spoilage of Shelf Stable Apple Juice. Journal of Food Protection. 73:1886-1890. 2010
- Biosynthesis and transcriptional analysis of thurincin H, a tandem repeated bacteriocin genetic locus, produced by Bacillus thuringiensis SF361. FEMS Microbiol Lett. 299:205-213. 2009
- Characterization of mundticin L, a class IIa anti-Listeria bacteriocin from Enterococcus mundtii CUGF08. . Applied and Environmental Microbiology. 75:5708-5713. 2009
- Inactivation of different strains of Escherichia coli O157:H7 in various apple ciders treated with dimethyl dicarbonate (DMDC) and sulfur dioxide (SO2) as an alternative method.. Food Microbiology. 1:8-15. 2009
- Influence of storage temperature and apple variety on patulin production by Penicillium expansum. Journal of Food Protection. 72:1030-1036. 2009
- Influence of storage temperature and apple variety on patulin production by Penicillium expansum. Journal of Food Protection. 72:1030-1036. 2009
- Isolation and characterization of a protective bacterial culture isolated from honey active against American Foulbrood Disease. FEMS Microbiol Lett. 296:39-44. 2009
- Modeling Penicillium expansum resistance to thermal and chlorine treatments. Journal of Food Protection. 72:2618-2622. 2009
- Antimicrobial activity of bacterial isolates from different floral sources of honey. . International Journal of Food Microbiology. 1-2:240-244. 2008
- Efficacy of sanitizing treatments against Penicillium expansum inoculated on six varieties of apples.. Journal of Food Protection. 3:643-647. 2008
- Purification and structural characterization of bacillomycin F produced by a bacterial honey isolate active against Byssochlamys fulva H25.. Journal of Applied Microbiology. 3:663-673. 2008
- Effects of Ultraviolet Irradiation on Chemical and Sensory Properties of Goat Milk. Journal of Dairy Science. 90:3178-86. 2007
- Effects of heat treatment on survival of Salmonella and E. coli O157:H7 on alfalfa seeds. Journal of Food Protection. 70:1698-703. 2007
- Comprehensive review of patulin control methods in foods. Comprehensive Reviews in Food Science and Food Safety. 4:8-21. 2005
- Efficacy of UV Light for the Reduction of Listeria monocytogenes in Goat’s Milk Intended for Cheese Production. Journal of Food Protection. 68:2212-2216. 2005
- Identification of the macromolecular synthesis operon of Oenococcus oeni. American Journal of Enology and Viticulture. 56:60-67. 2005
- Growth inhibition of foodborne pathogens and food spoilage organisms by select raw honeys. International Journal of Food Microbiology. 97:1-8. 2004
- Heat treatments to enhance the safety of contaminated mung bean seeds. Journal of Food Protection. 67:1257-1260. 2004
- Influence of apple cultivars on inactivation of different Strains of Escherichia coli O157:H7 in apple cider by UV irradiation. Applied and Environmental Microbiology. 70:6061-65. 2004
- Modeling of Escherichia coli: O157:H7 Inactivation by UV Irradiation at different pH Values in Apple Cider. Journal of Food Protection. 67:1153-1156. 2004
- Isolation, structural characterization, and properties of mattacin (polymyxin M), a cyclic peptide antibiotic produced by Paenibacillus kobensis M. Journal of Biological Chemistry. 278:13124-12132. 2003
- Purification and characterization of an antilisterial bacteriocin produced by Leuconostoc sp. W65. Journal of Microbiology and Biotechnology. 13:680-686. 2003
- Relationship among fecal coliforms and Escherichia coli in various foods. European-Food-Research-and-Technology. 216:331-334. 2003
- Storage quality of pasteurized and UV treated apple cider. J. Food Processing Preservation. 27:21-35. 2003
- Inactivation of Cryptosporidium parvum Oocysts in Fresh Apple Cider using Ultraviolet Irradiation. Appl. Environ. Microbiol. 68:4168-4172. 2002
- Purification and characterization of a bacteriocin (bovicin HC5) from Streptococcus bovis HC5. Microbiology. 148:3347-3352. 2002
- An Alternative Approach for Enumeration of Escherichia coli in Foods. International Journal of Food Microbiology. 68:217-223. 2001
- Cloning and Heterologous Expression of Xylanase from Pichia stipitis in Escherichia coli. Journal of Applied Bacteriology. 90:248-255. 2001
- E. coli O157:H7 as an Emerging Foodborne Pathogen: A Literature Review. Crit Rev Biotechnol. 21:27-48. 2001
- Analysis and Modeling of the Variability Associated with UV Inactivation of Escherichia coli in Apple Cider. Journal of Food Protection. 63:1587-90. 2000
- Characterization and purification of a bacteriocin produced by Lactobacillus acidophilus 30SC: Human isolate for potential use as a probiotic strain. Journal of Dairy Science. 83:2747-52. 2000
- Detection of Cholera Toxin Binding Activity of -Casein Macropeptide and optimization of its Production by use of Response Surface Methodology. Biosci Biotechnol Biochem. 64:516-22. 2000
- Genes Responsible for Production and Immunity of Carnobacteriocin A: the Immunity Protein Confers Cross-protection to Enterocin B. Microbiology. 146:621-31. 2000
- Kinetics of microbial inactivation for alternative processing technologies: Ultraviolet light. Journal of Food Science. 65:905-925. 2000
- Atypical Genetic Locus for Enterocin B Production in Enterococcus faecium BFE 900. Appl. Environ. Microbiol. 65:2170-2178. 1999
- E. coli O157:H7 as an Emerging Foodborne Pathogen: A Literature Review. Critical Reviews in Food Science and Nutrition. 39:481-502. 1999
- Alicyclobacillus: An Emerging Problem for New York’s Processors of Fruit Juices. Venture. 1:6-7. 1998
- Apple Cider Safety, Regulations and Processing Alternatives. New York State Fruit Quarterly. 6:13-16. 1998
- Apple cider: treatment options to comply with new regulations. J. Assoc. Food and Drug Offic. 62:19-26. 1998
- Choosing the Best Sanitizer for Your Needs. Venture. 1:3. 1998
- Food Safety and You, Top 10 Food Safety Questions and Answers. Venture. 1:3. 1998
- Heterologous expression of mesentericin Y105 using the general secretion pathway (GSP) or the dedicated transport system in Gram-positive and Gram-negative bacteria. Microbiology (Reading). 144:2845-2854. 1998
- Double-Glycine Type Leader Peptides Direct Secretion of Bacteriocins by ABC Transporters: Colicin V Secretion in Lactococcus lactis. Molecular Microbiology. 23:1293-1301. 1997
- Expression of the Antimicrobial Peptide Carnobacteriocin B2 by a Signal Peptide Dependent General Secretory Pathway. Appl. Environ. Micro. 62:4095-4099. 1996
- A Signal Peptide Secretion-dependent Bacteriocin of Carnobacterium divergens. Journal of Bacteriology. 177:3143-3149. 1995
- Heterologous Expression of the Lactacin F Peptides by Carnobacterium piscicola LV17. Appl. Environ. Microbiol. 61:1371-1377. 1995
- Characteristics and Genetic Determinant of a Hydrophobic Peptide Bacteriocin, Carnobacteriocin A, Produced by Carnobacterium piscicola LV17A. Microbiology (Reading). 4:517-526. 1994
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article
- Bribes let tomato vendor sell tainted foods. New York Times. . 2010
- Kombucha . Washington Post. . 2010
- Produce sanitizers-are they worth it? 2010
- Reduction of Patulin in Apple Cider by UV Radiation. Journal of Food Protection. 73:69-74. 2010
- Thermoaciduric Clostridium pasteurianum Spoilage of Shelf-Stable Apple Juice. Journal of Food Protection. 73:1886-1890. 2010
- Characterization of Mundticin L, a Class IIa Anti-Listeria Bacteriocin from Enterococcus mundtii CUGF08. Applied and Environmental Microbiology. 75:5708-5713. 2009
- A response to the article: Loving, Anne LaGrange; Perz, John. 2007 Microbial flora on restaurant beverage lemon slices 2008
- Sap Steady Ultraviolet Processing Unit for Maple Sap 2007
- Thermal inactivation of Salmonella and Escherichia coli O157 : H7 on alfalfa seeds. Journal of Food Protection. 70:1698-1703. 2007
- Thermal inactivation of Salmonella and Escherichia coli O157 : H7 on alfalfa seeds 2007
- Controlling microbial populations in sap systems. Maple Syrup Digest. 18A:24-27. 2006
- Ultraviolet as an alternative fruit juice processing method to meet FDA safety requirements 2005
- Ultraviolet as an alternative fruit juice processing method to meet FDA safety requirements 2005
- Unraveling the mystery of the activity in select honeys 2005
- Water activity: another critical factor for safety of food products 1999
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booksection
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chapter
- Decontamination of Juices. Microbial decontamination in the food industry: Novel methods and applications. 2012
- Juices and Functional Drinks. Specialty Foods: Processing Technology, Quality, and Safety. 239-71. 2012
- Fruit and Vegetable Microbiology. Processing Fruits: Science and Technology. 261-285. 2004
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conference paper
- Testing for pathogens in irrigation water.. Proceedings of the Mid-Atlantic Fruit and Vegetable Convention.. 114-115. 2012
- Assessing the Quality of Surface Water Utilized for Fresh Produce Production in New York. 2011 IAFP Annual Conference Proceedings. 2011
- Characterization of Self-lethality Phenotype of Mundticin L Producer, Enterococcus mundtii CUGF08. 2011 IAFP Annual Conference Proceedings. 2011
- Decontamination of Green Onions and Spinach Using Gaseous Ethyl Pyruvate . 2011 IAFP Annual Conference Proceedings. 2011
- Inhibitory effect of dimethyl dicarbonate on Alicyclobacillus acidoterrestris and physicochemical properties of apple juice . 2011 IFT Annual Conference Proceedings. 2011
- Pathogens in Produce Production Environments: A Longitudinal Study of Fruit and Vegetable Farms in New York State. 2011 IAFP Annual Conference Proceedings. 2011
- Postharvest Intervention Methods and Combined Treatments to Decontaminate Spinach. 2011 IAFP Annual Conference Proceedings. 2011
- Preliminary Results of Irrigation Water Sampling. Proceedings of the 2011 Fruit and Vegetable Expo. 2011
- Inactivation of E. coli O157:H7 on Green Onions. Proceedings of the Institute of Food Technologists Annual Meeting & Food Expo, Chicago, IL. 2010
- Isolation and Detection of Listeria monocytogenes , E. coli O157:H7, non-O157 shiga Toxin Producing E. coli and Salmonella from Produce Farms. Cornell University’s Infection and Pathobiology Annual Retreat, Owego, NY, October 2010. 2010
- Isolation of two bacteriocin-producing lactic acid bacteria from alfalfa seeds. 2008 IFT Program and Abstract Book Annual Meeting. 010-029. 2008
- Pinot noir grape polyphenolic extracts: Chemical composition and antimicrobial effects against Streptococcus mutans and Alicyclobacillus acidoterrestris.. 2008 IFT Program and Abstract Book Annual Meeting. 056-01. 2008
- Effect of dimethyl dicarbonate addition on the retention of water soluble vitamins in juice systems. IFT National Conference. 2007
- Purification and chemical characterization of polymyxin E produced by a bacterial isolate from honeybee sac active against Paenibacillus larvae subsp. Larvae. IFT National Conference. 2007
- UV processing of juice to meet regulatory requirements (Abstract C107). 231st American Chemical Society National Meeting. 2006
- Effects of heat treatment on survival of Salmonella spp. and E. coli O157:H7 on alfalfa seeds. IAFP Annual National Conference. 2005
- Juice HACCP – decontaminating juice/apple surfaces. Food safety from the surface up. 2005
- The Detection and Survival of Salmonella, Escherichia coli, and Listeria monocytogenes in Selected Pesticide Sprays for Use on Fresh Produce. IAFP 91st Annual Meeting Program and Abstract Book. 2004
- Factors related to microbial stabilization of acid fruit juices. 20th Annual Conference on the Responsibilities of Processing Specialists. 2000
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conference poster
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document part
- Differential attachment and biofilm formation by foodborne microorganisms on surfaces with nanoscale topography. 2012
- Efficiency of Ultraviolet Treatment of Apple Cider: Effects of Concentration of Suspended Insoluble Solids, and Enzymatic Browning. 2012
- Efficient reduction of E. coli from apple cider by combining microfiltration with ultraviolet treatment. 2012
- Pickled Egg Production: Inactivation Rate of Salmonella, Escherichia coli O157:H7, Listeria monocytogenes and Staphylococcus aureus during Acidification Step. 2012
- Ultraviolet Treatment of Pathogens in Surface Irrigation Water.. 17-18. 2012
- Nanoengineered surfaces for controlling the attachment of pathogenic and biofilm forming bacteria to food contact surfaces 2011
- Effect of Postharvest and Processing Methods on Niagara Grape Juice Quality 2010
- Shelf-life Evaluation of Natural Antimicrobials for Concord Grape Juice 2010
- Effects of dimethyl dicarbonate addition on the retention of water soluble vitamins in juice systems 2007
- Sushi rice formulation affects pH and water activity, and inhibits growth of Bacillus cereus 2005
- Antibacterially active honey used as a preservative in meats, fruits, and juices 2004
- Effect of ultraviolet treatment on ascorbic acid in apple juice 2004
- Antimicrobial activity exhibited by bacteria isolated from selected U.S. honey samples 2003
- Increasing the shelf-life of sweet apple cider without adding preservatives 2003
- Modeling of Escherichia coli: 0157:H7 inactivation by UV irradiation at different pH in apple cider 2003
- Antimicrobial activity of honey against food pathogens and food spoilage microorganisms 2002
- Quality and shelf-life of processed apple cider 2002
- Factors affecting survival of Escherichia coli in specialty mustards 2001
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manual
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report
- Cider Pasteurization. Response to “Pasteurization is no silver bullet”. 34. 2007
- The Great Debate: Treatment Success Is Proven. 12-13. 2007
- Unraveling the mystery of antibacterial activity in select honeys 2005
- Shelf life extension of maple cream. 20-31. 2004
- Maximum likelihood estimation for models of log-reduction values 2002
- Non-chlorine Sanitizer Options for Wineries 2005
- Small Scale Food Entrepreneurship: A Technical Guide for Food Ventures 2003
featured in archived article
Teaching
teaching activities
- FDSC-5000: Master of Professional Studies (Agriculture) Project - Spring 2013
- FDSC-6960: Graduate Internship in Food Science - Spring 2013
- FDSC-8900: Master's Level Thesis Research - Spring 2013
- FDSC-9900: Doctoral-Level Thesis Research - Spring 2013
- FDSC-5000: Master of Professional Studies (Agriculture) Project - Fall 2012
- FDSC-6960: Graduate Internship in Food Science - Fall 2012
- FDSC-6970: Graduate Individual Study in Food Science - Fall 2012
- FDSC-8900: Master's Level Thesis Research - Fall 2012
- FDSC-9900: Doctoral-Level Thesis Research - Fall 2012
- FDSC-5000: Master of Professional Studies (Agriculture) Project - Spring 2012
- FDSC-6960: Graduate Internship in Food Science - Spring 2012
- FDSC-8900: Master's Level Thesis Research - Spring 2012
- FDSC-9900: Doctoral-Level Thesis Research - Spring 2012
- FDSC-5000: Master of Professional Studies (Agriculture) Project - Fall 2011
- FDSC-6960: Graduate Internship in Food Science - Fall 2011
- FDSC-6970: Graduate Individual Study in Food Science - Fall 2011
- FDSC-8900: Masters-Level Thesis Research - Fall 2011
- FDSC-9900: Doctoral-Level Thesis Research - Fall 2011
Service
outreach overview
- My primary outreach is focused on the fruit, vegetable and beverage industries in New York State. I provide my expertise in sanitation, processing recommendations for products that have problematic heat resistant molds and bacteria spoilage as well as answering basic food safety questions. I am actively involved in Juice HACCP training for the juice industry in New York State as well as the rest of the US. My outreach program is accomplished through workshops, conferences and direct contact with food processors.
service to the profession
- Association of Food and Drug Officials Food Committee Committee Member 2005 -
- IFT Science Reports and Emerging Issues Committee Committee Member 2004 -
- Food and Drug Administration Juice HACCP Curriculum Committee Committee Member 2000 -
- American Society for Microbiology
- International Association of Food Protection
- IFT Food Microbiology Division Executive Committee Committee Member 2006 - 2009
- IAFP Developing Scientist Committee Committee Member 2002 - 2005
- IAFP Educator Award Committee Committee Member 2002 - 2005
Background
education and training
- Ph.D. in Food Microbiology, University of Alberta 1995
- University of Alberta 1990
awards and honors
- Grocery Manufacturers of America (GMA) Food Safety Award (team award to the Cornell Institute of Food Science food safety team), 2010
- Merrill Presidential Scholar Outstanding Educator, 2004
- CALS Award for Excellence in Mentoring Undergraduate Students in Independent Research, 2003
- Employer Recognition Award, 2003
- French Ministry of Foreign Affairs Postdoctoral Award, 1994
- Canadian Institute of Food Science & Technology Scholarship, 1990
Other
college
- CALS
research keyword
- Bacteriocins
- Food Safety
- Fruits and Vegetable Safety
- Juice HACCP
- Produce
- Ultraviolet Processing
name prefix
- Professor