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Wolfner, Mariana Federica
Professor and Stephen H. Weiss Presidential Fellow
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Mariana Federica Wolfner is a member of Cornell's Graduate Fields of Genetics, Genomics & Development, of Biochemistry, Molecular & Cell Biology, and of Ecology & Evolutionary Biology. Her research is focused on understanding, at the molecular/gene level, the important reproductive processes that occur around the time when a sperm fertilizes an egg. Using the Drosophila model, the Wolfner laboratory studies the molecular signals that "activate" an oocyte to initiate embryo development and also the actions of seminal proteins that female flies receive from the males with which they mate. She has published over 130 papers in peer-reviewed journals and has served on a number of NIH study sections, including current membership on CMIR. She is on the Editorial Boards of several journals, including Genetics and Molecular Reproduction and Development, and she is or has been an Officer of the Genetics Society of America and the AAAS. She has been given numerous honors for her research endeavors, including a Junior Faculty Research Award from the American Cancer Society, a Career Advancement Award from the National Science Foundation, a Lady Davis Fellowship, and election as a Fellow of the American Association for the Advancement of Science. She has also received awards from Cornell for teaching and advising (Stephen H. Weiss Fellow, Robert A. and Donna B. Paul Award, Stephen & Margory Russell Award, and Kendall Carpenter Award), and is a Hunter Rawlings faculty fellow at Cornell’s Alice Cook House.
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advisory group member
Research
research overview
- Biological research has always been a passion of mine. I love working with a team of talented students, postdocs and staff to uncover important biological facts and concepts. Specifically, we are interested in understanding, at the molecular/gene level, the important reproductive processes that occur around the time when a sperm fertilizes an egg. Most of our work uses the Drosophila fruit fly, because of its excellent genetics/genomics, the ease with which its molecular biology, development and reproduction can be studied, and its ability to serve as a model – because many gene products and reproductive/developmental phenomena in Drosophila have counterparts or analogues in other animals, including people, and insect pests. In recent years we have begun a collaborative study (with Laura Harrington) aiming to obtain results that can be applied to a practical goad: control of the reproduction of the dengue-vector mosquito. In one project we focus on the actions of seminal proteins that female flies receive from the males with which they mate. One of our first contributions to this field was to show genetically that male accessory gland (seminal) proteins (“Acps”) modify the behavior and reproductive physiology of the mated female. For example, they increase the female’s egg-production, make the female less likely to mate again, affect her feeding and sleep behaviors and her longevity, and cause her to store and maintain sperm that she received in the mating. Action of Acps can facilitate the efficiency or success of reproduction; we consider their action as improving the “ecology” in which the fertilization process occurs. We are examining at the molecular level how Acps accomplish this. As part of this investigation, we identified a large fraction of Acps by RNA-based and genomic methods, and showed the transfer and fate of a number of them to/in females. Using RNA interference (RNAi) we identified functions for specific Acps, in ovulation, sperm-storage-entry, sperm-storage maintenance, and in persistence of the post-mating changes in females. Our current research in this area aims to identify the molecular pathways through which Acps accomplish this. We have found, for example, that proteolytic processing of Acps (that we believe may regulate their function) is the result of a molecular pathway that has players from both the male and female; thus there is molecular synergy between the sexes. An extension of this area of work, in collaboration with our colleague L. Harrington, is to identify the Acps that control reproduction in the dengue vector mosquito. Using a variety of current methods we have identified almost 100 Acps in this species, and are using RNA interference (analogous to what we have done in Drosophila) to find those with reproductively-critical functions that could then be targets for vector-control programs. In addition to playing important reproductive roles, Acps (like a number of other important reproductive proteins) show unusual evolutionary dynamics that suggest that they may play important roles in reproductive isolation between species. We also study this aspect of Acps (in part, in collaboration with our colleagues A. Clark and C. Aquadro). In this aspect of our research we aim to gain insight into how forces like sperm storage, sexual conflict, female choice affect the expression, transfer and evolution of seminal proteins. In our other project, we study the molecular signals that “activate” an oocyte to initiate embryo development. An activated egg completes meiosis, fuses its genetic material with that of the sperm, and begins the mitotic (cleavage) divisions. The trigger for activation differs among organisms –in Drosophila we showed that this trigger occurs during ovulation – but the subsequent molecular changes often have commonality across organisms. Thus Drosophila provides a genetic model with which to dissect the changes that accompany and cause egg activation. We have shown that, as in animals that use sperm to activate their eggs, Drosophila eggs require calcium to activate, and (in collaboration with our colleague M. Goldberg) we identified a calcium-signaling pathway member that is essential for activation in Drosophila. Similarly, as in non-insects, we showed that levels of active MAPKs drop drastically in Drosophila eggs upon activation. Because of these commonalities, we are using Drosophila to identify new genes and molecular changes that mediate egg activation. For example we identified a poly-A polymerase that is needed to extend the poly-A tails of several mRNAs upon activation, to allow their translation into proteins for the embryo (we also found that this protein has a counterpart in the male, that is critical for the formation of functional sperm). We identified many proteins (such as the nuclear protein YA, which mediates the cell cycle transition from meiosis to mitosis) whose phosphorylation state changes dramatically upon egg activation. Our goal is to work out the regulatory cascades that translate the calcium signal into the production of new proteins for the early embryo or into post-translational modifications (such as specific protein phosphorylations) that allow embryogenesis to proceed.
research activities
principal investigator on
- ACTIONS OF SEMINAL PROTEINS IN MATED DROSOPHILA FEMALES awarded by NATL INST OF HEALTH DHHS 2010 - 2015
- FUNCTIONAL AND COEVOLUTIONARY ANALYSIS OF INTERACTING REPRODUCTIVE PROTEINS IN DROSOPHILA awarded by NATL INST OF HEALTH DHHS 2011 - 2014
- MOLECULAR GENETICS OF EGG ACTIVATION AND PRONUCLEAR FUNCTION IN DROSOPHILA awarded by NATL INST OF HEALTH DHHS 2007 - 2013
co-principal investigator on
- DROSOPHILA GENES CAUSING MALE X FEMALE INTERACTION IN REPRODUCTION awarded by NATL INST OF CHILD HEALTH & HUMAN DEVELOPMENT, NIH 2012 - 2017
- DROSOPHILA GENES CAUSING MALE X FEMALE INTERACTION IN REPRODUCTION: ARRA awarded by NATL INST OF HEALTH DHHS 2009 - 2012
- NEW TARGETS FOR REPRODUCTIVE CONTROL OF MOSQUITO VECTORS awarded by NATL INST OF HEALTH DHHS 2011 - 2016
keywords
- Drosophila
- RNA poly-adenylation
- calcium signaling
- early embryogenesis
- egg activation
- evolution (of reproductive proteins)
- fertility
- fertilization
- insect biology
- peptide hormones
- proteolysis
- reproduction
- seminal proteins
- sperm (insect)
submitted impact statement
Publications
individual publications
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academic article
- A Novel Function for the Hox Gene Abd-B in the Male Accessory Gland Regulates the Long-Term Female Post-Mating Response in Drosophila . PLoS Genetics. 9. 2013
- A large neurological component to genetic differences underlying female sperm preferences in Drosophila. Genetics. 193:177-85. 2013
- Calcium and egg activation in Drosophila. . Cell Calcium. 53:10-15. 2013
- Identification and function of proteolysis regulators in seminal fluid. . Molecular Reproduction and Development. 80:80-101. 2013
- Molecular changes during egg activation. Curr. Top. Devel. Bio.. 102:267-292. 2013
- Duration and dose-dependency of female sexual receptivity responses to seminal fluid proteins in Aedes albopictus and Ae. aegypti mosquitoes. Journal of Insect Physiology. 58:1307-1313. 2012
- Female Drosophila melanogaster suffer reduced defense against infection due to seminal fluid components.. Journal of Insect Physiology. 58:1192-1201. 2012
- Protein phosphorylation changes reveal new candidates in the regulation of egg activation and early embryogenesis in D. melanogaster.. Developmental Biology. 370:125-34. 2012
- Requirement for the neuromodulators octopamine and tyramine in D. melanogaster female sperm storage. . Proceedings of the National Academy of Sciences of the United States of America. 109:4562-7. 2012
- Temporally variable selection on proteolysis related reproductive proteins in Drosophila.. Molecular Biology and Evolution. 29:229-238. 2012
- The 2012 Thomas Hunt Morgan Award: Kathryn V. Anderson. Genetics. 191:293-5. 2012
- The Drosophila seminal fluid protease 'Seminase' regulates proteolytic and post-mating reproductive processes. PLoS Genetics. 8. 2012
- The Spermatid Individualization Complex of Drosophila melanogaster. in Visions, the art of science. Molecular Reproduction & Development. 79:367. 2012
- The consequences of genetic variation in male sex peptide gene-expression levels for SP retention and egg laying in female Drosophila.. Heredity. 109:222-5. 2012
- Therese Markow and the George M. Beadle Award. . Genetics. 191:299-300. 2012
- ¬Overview: Special Issue on Drosophila Spermatogenesis. {Dr. Brill and I co-edited this issue}. Spermatogenesis. 2:127-128. 2012
- Functional genome annotation of Drosophila seminal fluid proteins using transcriptional genetic networks. Genetics Research. 93:387-95. 2011
- Insect seminal fluid proteins: identification and function. Annual Reviews of Entomology. 56:21-40. 2011
- Male Drosophila melanogaster exploit specific seminal fluid proteins from the ejaculates of rival males¬. . Proceedings of the National Academy of Sciences of the United States of America. 108:9922-6. 2011
- Meet the Editors.. Molecular Reproduction & Development. 78. 2011
- Precious essences: female secretions promote sperm storage in Drosophila.. PLoS Biology. 9. 2011
- The poly(A) polymerase GLD2 is required for spermatogenesis in Drosophila melanogaster. Development. 138:1619-1629. 2011
- Towards a semen proteome of the dengue vector mosquito: Protein identification and potential functions. PLoS Neglected Trop. Diseases. 5. 2011
- Genetic interactions between the sexes mediate sperm competition in Drosophila: Sex Peptide × Sex Peptide Receptor. Genetics. 186:1355-65. 2010
- Immortal coils: conserved dimerization motifs of the Drosophila seminal prohoromone ovulin. Insect Biochemistry and Molecular Biology. 40:303-10. 2010
- Sex peptide is required for the efficient release of stored sperm in mated Drosophila females. . Genetics. 186:595-600. 2010
- The genetic basis for male x female interactions underlying variation in reproductive phenotypes of Drosophila. Genetics. 186:1355-65. 2010
- A network of interactions among seminal proteins underlies the long-term post-mating response in Drosophila. Proceedings of the National Academy of Sciences (PNAS). 106:15384-9. 2009
- Acp36DE is required for uterine conformational changes in mated Drosophila females. Proceedings of the National Academy of Sciences (PNAS). 106:15796-800. 2009
- Battle and ballet: molecular interactions between the sexes in Drosophila. Journal of Heredity. 100:399-410. 2009
- Molecular social interactions: Drosophila melanogaster seminal fluid proteins as a case study. Advances in Genetics. 68:23-56. 2009
- Seminal fluid protein allocation and male reproductive success. Current Biology. 19:751-7. 2009
- Strain-dependent differences in several reproductive traits are not accompanied by transcriptome changes in female Drosophila melanogaster. Genetics. 181:1273-80. 2009
- Tracking ejaculate dynamics: an ELISA-based method for quantifying seminal fluid proteins. . Behavioral Ecology and Sociobiology. 63:1505-1513. 2009
- A role for Acp29AB, a predicted seminal fluid lectin, in female sperm storage in Drosophila melanogaster. Genetics. 180:921-31. 2008
- Identity and transfer of male reproductive gland proteins of the dengue vector mosquito, Aedes aegypti: potential tools for control of female feeding and reproduction. Insect Biochemistry and Molecular Biology. 38:176-189. 2008
- Mechanical stimulation by osmotic and hydrostatic pressure activates Drosophila oocytes in vitro in a calcium-dependent manner. Developmental Biology. 316:100-109. 2008
- Post-mating gene expression profiles of female Drosophila melanogaster in response to time and to four male accessory gland proteins. Genetics. 179:1395-408. 2008
- Targeted gene deletion and phenotypic analysis of the Drosophila melanogaster seminal fluid protease inhibitor Acp62F. Genetics. 78:1605-1614. 2008
- Transitioning from egg to embryo: triggers and mechanisms of egg activation. Developmental Dynamics. 237:527-44. 2008
- Wispy, the Drosophila homolog of GLD-2, is required during oogenesis and egg activation. Genetics. 178:2017-29. 2008
- An ectopic expression screen reveals Drosophila seminal fluid proteins’ protective and toxic effects. Genetics. 175:777-83. 2007
- Effects of the larval rearing environment on post-copulatory traits in Drosophila melanogaster. Biology Letters. 3:607-610. 2007
- Evidence for Positive Selection on Drosophila melanogaster Seminal Fluid Protease Homologs. Molecular Biology and Evolution. 25:497-506. 2007
- Evidence of Amino Acid Diversity–Enhancing Selection within Humans and among Primates at the Candidate Sperm-Receptor Gene PKDREJ. American Journal of Human Genetics. 81. 2007
- Evolution in the fast lane: rapidly evolving sex-and reproduction-related genes in Drosophila species. Genetics. 177:1321-35. 2007
- Modulation of MAPK activities during egg activation in Drosophila. Fly. 1:222-227. 2007
- Seminal influences: Drosophila Acps and the molecular interplay between males and females during reproduction. Integrative and Comparative Biology. 47:427-445. 2007
- Seminal proteins, but not sperm, induce morphological changes in the Drosophila melanogaster female reproductive tract during sperm storage. Journal of Insect Physiology. 53:319-31. 2007
- Sustained post-mating responses in D. melanogaster require multiple seminal fluid proteins. PLoS Genetics. 3. 2007
- A predicted seminal astacin-like protease is required for the processing of reproductive proteins in Drosophila melanogaster. Proceedings at the National Academy of Science USA. 103:10674-10679. 2006
- Evidence for structural constraint on ovulin, a rapidly evolving Drosophila melanogaster seminal protein. Proceedings at the National Academy of Science USA. 103:18644-18649. 2006
- Sexual behavior: a seminal peptide stimulates appetites. Current Biology. 16. 2006
- The Drosophila Calcipressin Sarah Is Required for Several Aspects of Egg Activation. Current Biology. 16:1441-1446. 2006
- Cross-species comparison of Drosophila male accessory gland protein genes. Genetics. 171:131-143. 2005
- Emergence of sperm from female storage sites has egg-influenced and egg-independent phases in Drosophila melanogaster. Biology Letters. 2:128-130. 2005
- Fates and targets of male accessory gland proteins in mated female Drosophila melanogaster. Insect Biochemistry and Molecular Biology. 35:1059-1071. 2005
- Two cleavage products of the Drosophila Acp ovulin can independently induce ovulation. Proceedings at the National Academy of Science USA. 102:743-8. 2005
- Comparative structural modeling and inference of conserved protein classes in Drosophila seminal fluid. Proceedings at the National Academy of Science USA. 101:13542-7. 2004
- Evolutionary EST analysis of Drosophila female reproductive tracts identifies several genes subjected to positive selection. Genetics. 168:1457-1465. 2004
- Genes regulated by mating, sperm or seminal proteins in mated female Drosophila melanogaster. Current Biology. 14:1509-1514. 2004
- Seminal fluid and mating mediate changes in nerve termini innervating the Drosophila reproductive tract. Proceedings at the National Academy of Science USA. 101:6261-6266. 2004
- A hydrophilic lamin-binding domain from the Drosophila YA protein can target proteins to the nuclear envelope. Journal of Cell Science. 116:2067-2072. 2003
- An early role for Drosophila melanogaster male seminal protein Acp36DE in female sperm storage. Journal of Experimental Biology. 206:3521-3528. 2003
- Sex determination: Sex on the brain?. Current Biology. 13. 2003
- The developments between gametogenesis and fertilization: ovulation and female sperm storage in Drosophila. Developmental Biology. 256:195-211. 2003
- The sex peptide of Drosophila melanogaster: investigation of post-mating responses of females using RNA interference. Proceedings at the National Academy of Science USA. 100:9923-9928. 2003
- How to organize a flymeeting. . publication for the Drosophila Board. 2002
- Interaction of the essential Drosophila nuclear protein YA with P0/AP3 in the cytoplasm and in vitro: implications for developmental regulation of YA’s subcellular location. Developmental Biology. 244:429-441. 2002
- The Drosophila melanogaster seminal fluid protein Acp62F is a protease inhibitor that is toxic upon ectopic expression. Genetics. 160:211-244. 2002
- The Drosophila nuclear protein YA binds to DNA and histone H2B with four domains. Molecular Biology of the Cell. 13:558-569. 2002
- The gifts that keep on giving: physiological functions and evolutionary dynamics of male seminal proteins in Drosophila. Heredity. 88:85-93. 2002
- Another Thing They Never Taught You in Grad School: How to Organize a Scientific Meeting. . Science's Next Wave, http://nextwave.sciencemag.org/cgi/content/full/2001/05/10/2.. online. 2001
- Antibacterial activities made by the Drosophila melanogaster male accessory glands and ejaculatory duct are transferred to females during mating. Journal of Insect Physiology. 47:617-622. 2001
- Evolutionary EST analysis identifies rapidly evolving male reproductive proteins in Drosophila. Proceedings at the National Academy of Science USA. 98:7375-7379. 2001
- Fluorescing flies: examining protein targeting and insect reproductive behavior using Green Fluorescent Protein. Drosophila Information Service. 84:224-226. 2001
- Fluorescing flies: examining protein targeting and insect reproductive behavior using Green Fluorescent Protein. . Dros. Info. Serv.. 2001
- Identification of and characterization of the major D. melanogaster mating plug protein. Insect Biochemistry and Molecular Biology. 31:543-51. 2001
- Male contributions to egg production: the role of accessory gland products and sperm in Drosophila melanogaster. Proceedings of the Royal Society of London Series B. 268:175-180. 2001
- Ovulation triggers egg activation in Drosophila. Developmental Biology. 234:416-424. 2001
- Positive Darwinian selection in the evolution of mammalian female reproductive proteins. Proceedings at the National Academy of Science USA. 98:2509-2514. 2001
- The Acp26Aa seminal fluid protein is a modulator of early egg-hatchability in Drosophila melanogaster. Proceedings of the Royal Society of London Series B. 268:1647-1654. 2001
- The nuclear envelope: emerging roles in development and disease. Cell and Molecular Life Sciences. 58:1737-1740. 2001
- The nuclear envelope: emerging roles in development and disease. {introduction to an issue of CMLS that was co-reviewed by Dr. Wilson and me}. Cell & Molecular Life Sciences. 58:1737-40. 2001
- Offsetting effects of Wolbachia infection and heat shock on sperm production in Drosophila simulans: Comparative analyses of fecundity, fertility and accessory gland proteins. Genetics. 155:167-178. 2000
- The Drosophila seminal fluid protein Acp26Aa stimulates release of oocytes by the ovary. Current Biology. 10:99-102. 2000
- The role of male accessory gland protein Acp36DE in sperm competition in Drosophila melanogaster. Proceedings of the Royal Society of London Series B. 267:1097-1105. 2000
- Drosophila seminal fluid proteins enter the circulatory system through the walls of the posterior vagina. Insect Biochemistry and Molecular Biology. 29:1043-1052. 1999
- Male seminal fluid proteins are essential for sperm storage in Drosophila melanogaster. Genetics. 153:837-844. 1999
- Mated Drosophila females require a seminal fluid protein, Acp36DE, to store sperm efficiently. Genetics. 153:845-857. 1999
- Nuclear entry of the Drosophila melanogaster nuclear lamina protein YA correlates with developmentally regulated changes in its phosphorylation state. Developmental Biology. 210:124-34. 1999
- Wise, winsome or weird: Mechanisms of sperm storage in female animals. Current Topics in Developmental Biology. 41:67-97. 1999
- Functional dissection of YA, an essential, developmentally regulated nuclear lamina protein in Drosophila. Molecular and Cellular Biology. 18:188-197. 1998
- Interactions between Drosophila nuclear envelope proteins lamin otefin and YA. Molecular and Cellular Biology. 18:4315-4323. 1998
- Seminal fluid regulation of female sexual attractiveness in Drosophila melanogaster. Proceedings at the National Academy of Science USA. 95:4051-4054. 1998
- Developmental modulation of the nuclear envelope. Current Topics in Developmental Biology. 35:47-70. 1997
- Formation of the male pronuclear lamina in Drosophila. Developmental Biology. 184:187-196. 1997
- Mating and hormonal triggers regulate accessory gland expression in male Drosophila. Journal of Insect Physiology. 43:1117-1123. 1997
- New genes for male accessory gland proteins in Drosophila melanogaster. Insect Biochemistry and Molecular Biology. 27:825-834. 1997
- The Drosophila embryonic nuclear lamina protein "Young arrest" (fs(1)Ya) is capable of associating with chromatin. Journal of Cell Science. 110:643-651. 1997
- Tokens of love: functions and regulation of Drosophila male accessory gland products. Insect Biochemistry and Molecular Biology. 27:179-192. 1997
- Localization of the Drosophila male accessory gland protein Acp36DE in the mated female suggests a role in sperm storage. Insect Biochemistry and Molecular Biology. 26:971-980. 1996
- A Drosophila seminal fluid protein, Acp26Aa, stimulates egg laying in females for 1 day after mating. Proceedings at the National Academy of Science USA. 92:10114-10118. 1995
- Cost of mating in Drosophila melanogaster female is mediated by male accessory gland products. Nature. 373:241-244. 1995
- Male and female cooperate in the prohormone-like processing of a Drosophila melanogaster seminal fluid protein. Developmental Biology. 171:694-702. 1995
- Mutational analyses of fs(1)Ya, a developmentally regulated nuclear envelope protein in Drosophila. Genetics. 141:1473-1481. 1995
- The fs(1)Ya protein of Drosophila is a component of the lamina of cleavage nuclei, pronuclei and polar bodies in early embryos. Developmental Biology. 163:202-211. 1994
- The mysteries of development. (Book review of Wilkins' Genetic Analysis of Animal Development). . J. Heredity. 86:323-4. 1994
- Two tightly-linked Drosophila male accessory gland transcripts with the same developmental regulation derive from independent transcription units. Mechanisms of Development. 48:51-57. 1994
- Probing accessory gland function by directed cell ablation in Drosophila. Proceedings at the National Academy of Science USA. 90:8093-8097. 1993
- Cell-type specific gene expression in the Drosophila melanogaster male accessory gland. Mechanisms of Development. 38:33-40. 1992
- DNA Sequence requirements for generating paused polymerase at the start of hsp70. Genes & Development. 6:284-295. 1992
- Structure, expression and hormonal control of genes from the mosquito, Aedes aegypti, which encode proteins similar to the vitelline membrane proteins of Drosophila melanogaster. Developmental Biology. 155:558-568. 1992
- The view from the bottom: sex-specific traits and their control in Drosophila. Seminars in Developmental Biology. 3:331-340. 1992
- A maternally-encoded nuclear envelope protein required for embryonic mitosis in Drosophila. Cold Spring Harbor Symposia on Quantitative Biology. 56:719-727. 1991
- The Drosophila maternal effect gene fs(1)Ya encodes a cell cycle dependent nuclear envelope component required for embryonic mitosis. Cell. 64:49-62. 1991
- The doublesex locus of Drosophila melanogaster and its flanking regions: A cytogenetic analysis. Genetics. 127:125-138. 1991
- Parasites in Drosophila embryos. Nature. 348:117. 1990
- Structure, cell-specific expression and mating-induced regulation of a Drosophila melanogaster male accessory gland gene. Developmental Biology. 139:134-138. 1990
- Synthesis of two Drosophila male accessory gland products and their fate after transfer to the female during mating. Developmental Biology. 142:465-475. 1990
- Cloning and analysis of fs(1)Ya, a maternal effect gene required for the initiation of Drosophila embryogenesis. Molecular & General Genetics. 215:257-265. 1989
- A method for mass-isolating ecdysterone-inducible tissues of D. melanogaster. Drosophila Information Service. 67:104-106. 1988
- Determination of male-specific gene expression in Drosophila accessory glands. Developmental Biology. 126:195-202. 1988
- Localized heat shock induction in Drosophila melanogaster. Journal of Experimental Zoology. 247:279-284. 1988
- Molecular isolation and characterization of the dsx locus of D. melanogaster. Genes & Development. 2:477-489. 1988
- Sex-specific control of DrosophilaYP1 expression is limited to transcription. Molecular and Cellular Biology. 8:4756-4764. 1988
- Sex-specific gene expression in somatic tissues of Drosophila melanogaster. Trends in Genetics. 4:333-337. 1988
- Structure and expression of a Drosophila male accessory gland gene whose product resembles a peptide pheromone precursor. Genes & Development. 2:1063-1073. 1988
- Sequences expressed sex-specifically in D. melanogaster adults. Developmental Biology. 119:242-251. 1987
- The vitellogenin gene family of Aedes aegypti. Memórias do Instituto Oswaldo Cruz. 82 Supplement III:109-114. 1987
- Mosquito vitellogenin genes: isolation and induction by 20-hydroxyecdysone. Insect Biochemistry. 16:761-774. 1986
- Spatial and temporal pattern of hsp26 expression during normal development. EMBO Journal. 5:747-754. 1986
- Control of sexual differentiation in Drosophila melanogaster. Cold Spring Harbor Symposia on Quantitative Biology. 50:605-614. 1985
- Sex-specific regulation of yolk protein gene expression in Drosophila. Cellular Immunology. 40:339-348. 1985
- Yeast suppressors of UAA and UAG nonsense codons work efficiently in vitro via tRNA. Cell. 7:381-390. 1976
- Integration of amino acid biosynthesis into the cell cycle of Saccharomyces cerevisiae. Journal of Molecular Biology. 96:273-290. 1975
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article
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booksection
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chapter
- Ejaculate- and sperm-female interactions. Sperm Evolution. 2009
- “S.P.E.R.M.” (Seminal Proteins (are) Essential Reproductive Modulators): the view from Drosophila. Spermatology. 183-199. 2006
- Insect gonadal glands and their gene products. Comprehensive Insect Physiology, Biochemistry, Pharmacology and Molecular Biology. 179-212. 2005
- Nuclear envelope dynamics in Drosophila pronuclear formation and in embryos. Dynamics of Nuclear Envelope Assembly in Embryos and Somatic Cells. 2001
- Harnessing the power of Drosophila genetics. Methods in Cell Biology Volume 4. Drosophila melanogaster: Practical uses in cell and molecular biology. 34-80. 1994
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conference paper
- Molecular genetics of sex determination in Drosophila. Gametogenesis and the Early Embryo: Society for Developmental Biology Symposium 44. 3-17. 1986
- Sex determination in Drosophila melanogaster. Genetics: New Frontiers, Proceedings of the XV International Congress of Genetics, Volume III, Genetics and Health. 223-232. 1983
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review
featured in archived article
Teaching
teaching overview
- I consider teaching – in-class, in the research lab, and through other informal interactions – to be an extremely important way in which I can contribute professionally (and, I love it!). My goal is to transmit to students the excitement of biology, and the joy in uncovering and interrelating biological concepts and facts. In all of my teaching I strive to work with students as individuals (this is easy in the lab and informal setting, but I try to do so in my classroom teaching as well). Classroom teaching: I strive to bring to the classroom the latest fields, findings and theories in molecular developmental biology, including their intersections with genetics and with evolution. I modify my courses extensively each year, to keep up with these fast-moving fields. I also have, not infrequently, developed new courses or taken over other courses, in order to keep our course-offerings congruent with new and exciting areas in the field-as-a-whole, or to keep our curriculum focused on the most important areas (related to this, I played a central role in the redesign of the overall biology major curriculum). I’ve taught additional courses, to bring topics such as genomics of non-model organisms, or sperm storage patterns, to the community. I have always enjoyed thinking about – and thus teaching at – the novel approaches and results that occur at the intersection between fields. Thus, although my main teaching involves full-semester advanced courses (for which I am sole-instructor), some of my additional teaching has been in cross-disciplinary courses that I’ve taught in collaboration with faculty from other departments. My goal is to bring the latest information to the students, while still covering needed basics: in each lecture we move from very basic to current concepts. I use papers from the current literature as major resources; part of my goal is to get students comfortable with reading (and critiquing) scientific literature. I try to have each class be a conversation, a give-and-take in which students feel comfortable asking and answering questions. I like to integrate what I am teaching with what students have learned in other courses; I try to think in terms of our course being a ‘capstone’ for the general areas that we cover. I encourage curiosity from the students (and if I don’t know the answer to a student’s question, I find out and let them know in the next class). My goals are that students gain a working knowledge of the topic covered, including details of facts and approaches, molecular pathways and their discovery, integration and mechanisms. I also try to develop in my students the ability to interpret and analyze critically, current and “classic” ideas, and to communicate their thoughts orally and in writing. Where possible I use visual aids (beyond Powerpoints; e.g. I use specimens from museum-collections etc.) to illustrate my points. I use a variety of assessment tools to accommodate different learning styles: problem sets, figure-presentation, in-class discussion, in-class exams and quizzes, take-home finals. I make it clear to students that I’m happy to discuss materials outside of class as well as in class. Before the past 5 years, most of my major teaching was in two spring courses, that alternated in Springs: I was sole-instructor for “Advanced Developmental Biology” (BioGD483, a course that I developed) and for “Fertilization and the Early Embryo” (BioGD682, a course that I developed). In the past 5 years, my course-portfolio completely changed. I now alternate “Development and Evolution (BioMG4610, a course that I developed; first taught Spring ‘07) with “Developmental Genetics” (BioGD6870, a course developed by Ken Kemphues, that I took over in Spring ’10 when Dr. Kemphues’ teaching responsibilities changed). Here too, I have sole-responsibility for each of these full-semester courses. In the past decade I also have taught, or co-taught graduate (700-level) courses on EvoDevo, Genomics of Non-Model Organisms, Molecular Neurobiology, Sperm Use Patterns; these brought new areas to Cornell. In most of these courses we developed a new format in which students read papers from a scientist who then came and presented a talk to Cornell (and a second talk to (and interaction with) our students); this format has now been adopted by the Field of Genetics and Development for its BioGD7800 course. I participate annually in (and led for several years) the Genetics & Development paper-based “Problems in Genetics and Development” course, and occasionally in the Biochemistry, Cell and Molecular Biology version of this course. I lecture annually (or each time it is offered) in Macroevolution, Scientific Communication and Careers in BMCB, and Model Genetic Organisms courses; I guest-lecture in other courses as well. One-on-one, in-laboratory teaching: Undergraduate: I mentor ~3-5 undergraduates in research every semester. This involves close and individualized one-on-one teaching that I consider to be as important as my classroom teaching. Generally, undergraduates do research in my lab for ~4-6 semesters. I choose students who are interested and motivated, and together we discuss the lab’s research and select a feasible project that is of interest to the student. Each undergraduate is assigned a mentor from among the postdocs or more senior graduate students in my lab. The in-lab mentor’s project area is close to (but not identical) to the undergraduate’s, so that the mentor can work with the student to show him/her the needed techniques. I meet with each undergraduate in my lab at least once a week to discuss experiments, concepts, results, plans; I also coordinate with their graduate student or postdoc in-lab mentor. My goal is to teach the students how research is done, how controlled experiments are designed and interpreted, what it is like to do research and, of course, the basic theory and findings of our field and of the genetic, molecular or computational methods they use. Our undergraduates attend our labmeetings, and are encouraged to ask questions. They also present their research informally once a month and formally at the end of each semester. After their first semester in the lab, I assess the independence and originality of each student and give them further ‘ownership’ of their project if it is clear that they can handle this. In quite a few cases this has led to undergraduate co-authorship on scientific papers from our lab. Graduate: Laboratory ‘teaching’ of graduate students is more of a mentoring relationship, and is a more intensive and higher-level version of the undergraduate teaching described above. Among the higher expectations are that graduate students will be mature, creative independent colleagues on their projects by their 3rd or 4th year in graduate school. I view my role as being a supportive guide, helping them craft projects, critique results, work in a team and alone, find collaborators, present their results etc. I work with them on their project design and interpretation as a partner, not a “boss”. Informal teaching: I look for opportunities to talk with and teach students about biology, wherever I can. This can include participating in scientific interactions at Alice Cook House (where I’m a House Fellow; ‘interactions’ can be informal one-on-one, or my presenting slightly more formally to students in reading groups or in talks), or to first year students in various courses or in informal gatherings at the OUB, or to student groups, etc. I also make it clear to students in my classes (and to my undergraduate and graduate (minor and major) advisees) that I am available to talk with them about any topic related to biology (or biology careers if they wish).
teaching activities
Service
outreach overview
- Although I have no formal extension responsibilities, I routinely carry out several extension activities each year, including presentation to teachers, students, and other members of the lay public, providing Drosophila strains to local teachers and photographs of Drosophila reproductive structures to a museum for exhibit
service to the profession
- Center for Creative Leadership’s Leadership training for senior women faculty Trainee 2008 -
- Organizing Comm. Committee Member 2001 -
- AI-SPER-0203DF692AB000108EF Chairperson 1993 -
- American Association for the Advancement of Science
- International Society for Developmental Biology
- Sigma Xi
- Universities, institutes and other agencies (USDA) worldwide
- Genetics Soc. of America Committee Member 2012 - 2014
- Genetics Soc. of America Committee Member 2010 - 2014
- Genetics Soc. of America Officer, Secretary 2010 - 2013
- Genetics Soc. of America Committee Member 2010 - 2013
- Genetics Soc. of America Committee Member 2010 - 2012
- Genetics Soc. of America Member 2010 - 2012
- Amer. Assn. for Adv. of Science Poster-judge @ National Meeting 2008 - 2010
- AAAS Section G (Bio. Sci) Chairperson 2007 - 2010
- Amer. Assn. for Adv. of Science -- Chair, Section G (Biol. Sci.) Chairperson 2007 - 2010
- Genetics Society of America Board of Directors 2006 - 2009
- Larry Sandler Lectureship Committee Chairperson 2007 - 2008
- Larry Sandler Lectureship Committee Committee Member 2006 - 2008
- Board’s Subcommittee on Education/Outreach, Genetics Society of America Chairperson - 2008
- 2005 Siemens-Westinghouse competition National Judge - 2005
- Elections Committee, National Drosophila Board Committee Member - 2004
- 42nd Annual Drosophila Meeting Chairperson 2001
- Larry Sandler Lectureship Committee Chairperson 1993 - 1994
- Larry Sandler Lectureship Committee Committee Member 1991 - 1994
reviewer or editor for
- "Permanent" member of the CMIR study section
- CIFAR, review panel
- CMIR Study Section
- HHMI:Predoctoral Fellowships Panel
- HHMI:Predoctoral Fellowships Panel
- HHMI:Predoctoral Fellowships Panel
- NCF Study Section
- NICHD Special-Emphasis Study Section (Reproduction, Andrology, and Gynecology)
- NIH BIOL-1 Study Section
- NIH Genetics Study Section
- NIH Reviewers' Reserve
- NIH Special Emphasis Genetics Study Section
- NIH Special Genetics Study Sections, and AREA Grants
- NIH ZRG-1 Study Section
- NIH ZRG-1/NCF
- NIH ZRG-1/NCF
- NIH ZRG-1/NCF
- NIH/CMIR Study Section
- NIH/NICHD Special Study Section (Chair)
- NIH/NICHD Special Study Section (Chair)
- NIH: CMIR Study Section
- NIMH Cell, Developmental and Molecular Neurobiology Study Section, 1995-1997
- NSF Developmental Mechanisms Panel
- NSF Integrative Animal Biology Panel
- NSF Research Opportunities for Women Grants Panel
- NSF: ad hoc for numerous programs including (partial list): Animal Developmental Mechanisms, Eukaryotic Genetics, Ecological and Evolutionary Physiology, Visiting Professorships for Women, Integrative Animal Biology, Population Biology, Integrative Animal Biology, and Instrumentation Programs
- Rothschild Prize
- USDA: Entomology/Nematology
- USDA: Hatch
event host
Background
education and training
- Ph.D. in Biochemistry, Stanford Univ. 1981
- B.A. in Biology (Genetics & Development) & Chemistry, Cornell Univ. 1974
awards and honors
- Distinguished Lecture, 2012
- Kendall S. Carpenter Memorial Award, 2012
- Nelson Lecture, 2010
- Lady Davis Fellow, 2010
- Stephen and Margery Russell Award for Distinguished Teaching, 2009
- Wilhelmine Key Lecturer, 2008
- Robert A. And Donna B. Paul Award for Excellence in Advising, 2006
- Fellow, 2006
- Stephen H. Weiss Presidential Fellow, 2003
- Mentor Recognized by Cornell Merrill Scholar J. Reedy, 2000
- POWRE Award, 1997
- Mentor Recognized by Cornell Merrill Scholar A. Hirshfeld, 1992
- Faculty Research Award, 1989
- Career Advancement Award, 1988
- Mentor Recognized by Cornell Merrill Scholar K. Hanson, 1986
- Basil O'Connor Starting Faculty Scholar, 1985
- Du Pont Young Faculty Award, 1983
- Senior Postdoctoral Fellow, 1983
- Young Investigator Grant, 1983
- Postdoctoral Fellow, 1981
Other
college
- CALS
research keyword
- Drosophila
- RNA poly-adenylation
- calcium signaling
- early embryogenesis
- egg activation
- evolution (of reproductive proteins)
- fertility
- fertilization
- insect biology
- peptide hormones
- proteolysis
- reproduction
- seminal proteins
- sperm (insect)